Selected Publications From the Macromolecule and Vaccine Stabilization Laboratory Since 2001
(2007) Fang, J., Dong, Y., Salamat-Miller, N. And Middaugh, C.R. DB-PABP: a database of polyanion-binding proteins. Nucleic Acids Research. in press.
(2007) Kissmann J., Ausar, S.F., Foubert, T. A., Brock, J., Switzer, M.H., Detzi, E.J., Vedvick, T.S., and l Middaugh, C. R. Physical Stabilization of Norwalk Virus-Like Particles. J.Pharm.Sci. in press.
(2007) Salnikova, M.S., Joshi, S.B., Rytting, J.H., Warny, M and Middaugh, C.R. Preformulation Studies of Clostridium difficile Toxoids A and B. J. Pharm Sci in press.
(2007) Kamerzell, T.L., Ramsey, J and Middaugh, C.R. A statistical mechanical view of immunoglobulin dynamics. J. Phy. Chem., in press.
(2007) Salnikova, M., Joshi, S.B., Rytting, J.H., Warny, M., and Middaugh, C.R. Physical characterization of clostridium difficile toxins and toxoids-effect of formaldehyde crosslinking on thermal stability. J. Phar. Sci., in press.
(2007) Nonoyama, A., Lawrence, J. Garriques, L., Hong, Q., Thao, L and Middaugh, C.R. A biophysical characterization of the peptide drug Pramlintide (AC137) using empirical phase diagrams. J. Pharm. Sci, in press.
(2007) Birket, S.E., Harrington, A.T., Espina, M., Smith, N.D., Terry, C.M., Darboe, N., Markham, A.P., Middaugh, C.R., Picking, W.L. and Picking, W.D. Preparation and characterization of traslocator/chaperone complexes and their proteins from shigella flexineri. Biochemistry, 46, 8128-8137.
(2007) Kamerzell, T., and Middaugh, C.R. Two dimensional correlation spectroscopy reveals coupled immunoglobin regions of differential flexibility and Influence stability. Biochemistry, 46, 9762-9773.
(2007) Barrett, B.S., Picking, W.L., Picking, W.D., and Middaugh, C.R. The response of the TTSS needle proteins MxiHdelta5, BsaLdelta5 and PrgIdelta5 to temperature and pH. Submitted to Proteins: Struct., Funct., Proteomics.
(2007) Markham, A.P, Birket, S.E., Picking, W.D., Picking, W.L. and Middaugh, C.R. pH sensitivity of Type III secretion tip proteins. Proteins: Struct., Funct., Proteomics, in press.
(2007) Zheng, K., Lawrence, J.S.., Kuczera, K., Middaugh, C.R., and Siahaan, T.J. Characterization of multiple stable conformers of the fifth extracellular domain of E-cadherin. Submitted to J. Biol. Chem.
(2007) Middaugh, C.R. and Ramsey, J.D. Analysis of cationic lipid/plasmid DNA complexes. Anal Chem, 79(19), 7240-7248.
(2007) Joshi, S.B., Kamerzell, T., McNown, C.W. and Middaugh, C.R. Interaction of polyanions with bovine, porcine and human growth hormones: Effect on physical stability of these proteins. J. Pharm Sci, in press.
(2007) Kamerzell, T., Joshi, S.B., McClean, D., Peplinskie, L., Toney, K, Papac, D., Li, M., and Middaugh, C.R.. Parathyroid Hormone is a Heparin/Polyanion Binding Protein:Binding Energetics and Structure Modification. Protein Science. 16,1193-1203.
(2007) Brandau, D.T., Joshi, S.B., Smalter, A.M., Kim, S., Steadman, B and Middaugh, C.R. Stability of the Clostridium botulinum type A neurotoxin Complex: An Empirical Phase Diagram Based Approach. Mol. Pharm, 2007, 10.1021/mp0601244.
(2007) Ausar, S.A., Joshi, S.B. and Middaugh C.R. Methods for the Physical Characterization and Formulation of Nonviral Gene Delivery Systems. Methods in Molecular Biology: Gene Therapy Protocols, 3rd Edition (LeDoux J., Ed.), Humana Press, Totowa, NJ.
(2007) Ausar, S.F., Espina, M., Brock, J., Thyagrajapuram, N., Repetto, R. Khandke, L. and Middaugh, C.R. High through put screening of stabilizers for respiratory syncytial virus:identification of stabilizers and their effects on the formational thermostability of viral particles. Human vaccines. In press.
(2007) Li, Q.S., Cai, S., Borchardt, r.T., Fang, J., Kuczera, J., Middaugh, C.R. and Schowen, R.L. Comparative kinetics of cofactor association and dissociation for the human and trypanosomal S-adenosylhomo cysteine hydrolases. 1. basic features of the association and dissociation processes. Biochemistry, 46(19), 5798-5809.
(2007) Thyagrajapuram, N., Olsen, D. and Middaugh C.R. Stabilization of proteins by recombinant human gelatins. J. Pharm. Sci., 10.1002/jps.20980.
(2007) Thyagrajapuram, N., Olsen, D. and Middaugh C.R. The structure stability and complex behavior of recombinant human gelatins. J. Pharm. Sci., 2007, 10.1002/jps.21022.
(2007) Espina, M., Ausar, S.F., Middaugh, C.R., Baxter, M.A., Picking, W.D., and Picking, W.L. Conformational stability and differential structural analysis of LcrV, PcrV, BipD and SipD from Type III secretions systems. Protein Sci., Apr 2007, 16:704-714.
(2007) Salamat-Miller N., Fang J., Seidel C., Smalter A., Assenov Y., Albrecht M., and Middaugh C.R. A network-based analysis of polyanions binding proteins utilizing human protein arrays. J. Biol.Chem., in press.
(2007) Tian F. Middaugh C.R., Offerdahl T., Munson E., Sane S., Rytting J.H. Spectroscopic evaluation of the stabilization of humanized monoclonal antibodies in amino acid formulations. Int. J. Pharm., 2007 Apr 20, 335(1-2):20-31.
(2007) Fan H., Li H., Zhang M., Middaugh C.R. Effects of solutes on empirical phase diagrams of human fibroblast growth factor 1. J. Pharm. Sci., 2007, 10.1002/jps.20796.
(2007) Peek L.J., Martin T., Elk Nation C., Pegram S., Middaugh C.R. Effects of stabilizers on the destabilization of proteins upon adsorption to aluminum salt adjuvants. J Pharm. Sci., 2007 Mar, 96(3):547-57.
(2007) Harn N., Allan C., Oliver, C. Middaugh C.R. Highly Concentrated Monoclonal Antibodies: Direct Analysis of Structure and Stability. J. Pharm. Sci., 2007 Mar, 96(3):532-46.
(2007) Peek L.J., Brey R.N., Middaugh C.R. A rapid, three-step process for the preformulation of a recombinant ricin toxin A-chain vaccine. J. Pharm. Sci., 2007 Jan, 96(1):44-60.
(2007) Priddy, T.S., Middaugh, C.R. and Carlson, G.M. Electrostatic changes in phosphorylase kinase induced by its obligatory allosteric activator Ca2+. Protein Sci.,16, 517-527.
(2007) Derrick T., Grillo A.O., Vitharana S.H., Jones L.S., Rexroad J., Shah A., Perkins M., Spitznagel T. M., Middaugh C.R. Effect of polyanions on the structure and stability of Repifermin? (keratinocyte growth factor-2). J. Pharm. Sci, 5, 2263-2278
(2006) Kamerzell, T.J., Unruh, J.R., Johnson, C.K., and Middaugh, C.R. Conformational flexibility, hydration and state parameter fluctuations of fibroblast growth factor-10:effects of ligand binding. Biochemistry, 45, 15288-15300
(2006) Salamat-Miller N. Fang J. Seidel C. Smalter A. Assenov Y. Albrecht M. Middaugh C.R. A network-based analysis of polyanions binding proteins utilizing yeast protein arrays. Mol. Cell. Proteomics, 2006 Dec, 5(12):2263-78.
(2006) D’Souza A.M.J., Hart D.S., Middaugh C.R., Gehrke S.H. Characterization of he changes in secondary structure and architecture of elastin-mimetic triblock polypeptides during thermal gelation. Macromolecules, in press.
(2006) Lucas L.H., Ersoy B. A., Kueltzo L.A., Joshi S.B., Brandau, D.T., Thyagarajapurum N., Peek L.J., Middaugh C.R. Probing protein structure and dynamics by second derivative ultraviolet absorption analysis of cation-? interactions. Protein Sci, 2006 Oct, 15(10):2228-43.
(2006) Guo J., Harn N., Robbins A., Dougherty R., Middaugh C.R. Stability of helical-rich proteins at high concentration. Biochemistry, 45(28), 8686-96.
(2006) Peek L.J., Brandau D.T., Jones L.S., Joshi S.B.,Middaugh C.R. A systematic approach to stabilizing EBA-175 RII-NG for use as a malaria vaccine. Vaccine, 24(31-32), 5839-5851.
(2006) Espina M, Olive AJ, Kenjale R, Moore DS, Ausar SF, Kaminski RW, Oaks EV, Middaugh CR, Picking WD, Picking WL. IpaD localizes to the tip of the type III secretion system needle of Shigella flexneri). Infection and Immunity, 74(8), 4391-4400.
(2006) Espina M, Ausar SF, Middaugh CR, Picking WD, Picking WL. Spectroscopic and calorimetric analyses of IpaD from Shigella flexneri reveal the presence of two structural domains. Biochemistry 45(30), 9219-9227
(2006) Ausar S.F., Foubert T.R., Hudson M.H., Vedvick T.S., Middaugh C.R. Conformational stability and disassembly of Norwalk virus like particles: Effect of pH and temperature. J. Biol. Chem. 281(28), 19478-19488.
(2006) Ruponen M., Braun C.S., and Middaugh C.R. Biophysical characterization of polymeric and liposomal gene delivery systems using empirical phase diagrams. J Pharm Sci., 95(10), 2101-2114.
(2006) Rexroad, J., Martin, T.T., McNeilly, D., Godwin, S. and Middaugh, C.R. Thermal stability of Adenovirus 2 as a function of pH. J. Pharm. Sci., 95(7), 1469-1479.
(2006) Darboe, N., Kenjale, R., Picking, W.L., Picking, W.D., and Middaugh, C.R. Chemical denaturation of MxiH from Shigella and PrgI from Salmonella. Prot. Sci., 15, 543-552.
(2006) Fan, H., Kashi, R.S. and Middaugh, C.R. Conformational lability of two molecular chaperones HSc 70 and GP 96: Effects of pH and temperature. Arch Biochem. Biophys., 447, 34-45.
(2006) Harrington, A.T., Darboe, N., Picking, W.L., Middaugh, C.R., Birket, S., and Picking, W.D. Characterization of the interaction of a single tryptophan containing mutants of IpaC from Shigella flexneri with phospholipids membranes. Biochemistry, 45(2),626-636.
(2006) Purohit, V.S., Middaugh, C. R. and Balasubramanian, S.V. Influence of aggregation on the immunogenicity of recombinant human factor VIII in hemophilia mice. J. Pharm. Sci. 95, 358-371.
(2006) Jiang, G., Joshi, S. B., Peek, L. J., Brandau, D. T., Huang, C. R., Ferriter, M. S., Woodley, W. D., Ford, B. M., Mar, K. D., Mikszta, J. A., Hwang, C. R., Ulrich, R., Harvey, N. G., Middaugh, C. R. and Sullivan, V. J. Anthrax vaccine powder formulations for nasal mucosal delivery. J. Pharm. Sci. 95, 80-96.
(2005) Ausar, S.F., Rexroad, J., Frolov, V.G., Look, J.L., Konar, N. and Middaugh, C.R. Analysis of the thermal stability of respiratory syncytial virus. Mol. Pharm. 2, 491-499
(2005) Tumanova, I., Boyer, J., Ausar, S.F., Burzynski, J., Rosencrance, D., White, J., Schiedel, J., Parkinson, R., Maguire, H., Middaugh, C.R., Weiner, D., and Green, A. P. Analytical and biological characterization of supercoiled plasmids purified by various chromatographic techniques. DNA and Cell Biol 24,819-831.
(2005) Harn, N.R., Jeng, Y.N., Kostelec, J. C., and Middaugh, C.R. Spectroscopic analysis of highly concentrated suspensions of bovine somatotropin in sesame oil. J. Pharm. Sci., 94,2487-2497.
(2005) Fan, H., Ralston, J., DiBase, M., Faulkner, E., and Middaugh, C.R., The solution behavior of IFN-ß-1a: An empirical phase diagram approach. J Pharm. Sci., 94, 1893-1911.
(2005) Rexroad, J., Evans, R. K., and Middaugh, C. R. Effect of pH and ionic strength on the physical stability of adenovirus type 5. J. Pharm. Sci., 95,237-247.
(2005) Ramani, K., Purohit, V., Middaugh, C.R., and Balasubramauian, S.V. Aggregation kinetics of recombinant human FVIII (rFVIII). J. Pharm. Sci. 94, 2023-2029.
2005) Ramani, K., Purohit, V.S., Miclea, R.D., Middaugh, C.R. and Balasubramanian, S.V., Lipid binding region (2303-2332) is involved in aggregation of recombinant human factor VIII(rFVIII), J. Pharm. Sci. 94, 1288-1299.
(2005) Krogmeier, S.L., Reddy, D.S., Vander Velde, D., Lushington, G.H., Siahaan, T.J., Middaugh, C.R., Borchardt, R.T., and Topps, E.M. “Deamidation of Model [(beta)]—Turn Cyclin Peptides in the Solid State.” J. Pharm. Sci., 94, 2616-2631.
(2005) Ramani, K., Purohit, V.S., Miclea, R.D., Middaugh, C.R. and Balasubramanian, S.V. “Lipid Binding Region (2303-2332) is involved in Aggregation of Recombinant Human FVIII (rFVIII).” J. Pharm Sci., 94, 1288-1299.
(2005) Kueltzo, L.A. and Middaugh, C.R. “Molten Globule-Lipid Interactions and Their Implications for Protein Transport and Aggregation” in Protein (Mis)Folding, Aggregation and Stability (Tsai, A. and Murphy, R.M., eds.), Kluwer Academic Press.
(2005) Braun, C.S., Fisher, M.T., Tomalia, D.T., Koe, G.S., Koe, J.G., and Middaugh, C.R. “A Stopped-Flow Kinetic Study of the Assembly of Nonviral Gene Delivery Complexes,” Biophys. J., 88, 4146-4158.
(2005) Kueltzo, L.A. and Middaugh, C.R. “Polycationic Peptides and Proteins in Drug Delivery” in Drug Delivery: Principles and Applications (Wang, B., Siahaan, T. and Soltero, R., eds.), John Wiley & Sons.
(2005) Jones, L.S., Kaufmann, A. and Middaugh, C.R. “Silicone Oil Induced Aggregation of Proteins,” J. Pharm. Sci., 94, 918-927.
(2005) Jones, L.S., Peek, L.J., Power, J., Markham, A., Yazzi, B. and Middaugh, C.R. “Effects of Absorption to Aluminum Salt Adjuvants on the Structure and Stability of Model Protein Antigens,” J. Biol. Chem., 280, 13406-13416.
(2005) Braun, C.S., Vetro, J.A., Tomalia, D.A., Koe, G.S., Koe, J.G. and Middaugh, C.R. “Structure/Function Relationships of Polyamidoamine/DNA dendrimers as Gene Delivery Vehicles,” J. Pharm. Sci., 94, 423-436.
(2004) Derrick, T.S., Kashi, R.S., Durrani, M., Jhingan, A. and Middaugh, C.R. “Effect of Metal Cations on the Conformation and Inactivation of Recombinant Human Factor VII (rhFVIII)”, J. Pharm. Sci., 93, 2549-57.
(2004) Kueltzo, L.A. and Middaugh, C.R. “Ultraviolet Absorption Spectroscopy” in Methods for Structural Analysis of Protein Pharamceuticals (Jiskoot, W. ed.) AAPS Publications.
(2004) Jones, L.S., Yazzi, B. and Middaugh, C.R. “Polyanions and the Proteome”, Mol. Cell Proteomics, 3, 746-769.
(2004) Stotz, C.E., Borchardt, R.T., Middaugh, C.R., Siahaan, T.J., Vander Velde, D. and Topp, E.M. “Secondary Structure of a Dynamic Type I’ Beta Hairpin Peptide,” J. Peptide Res., 63, 371-82.
(2004) Wiethoff, C.M., Koe, J.G., Koe, G.S. and Middaugh, C.R. Compositional Effects of Cationic Lipid/DNA Delivery Systems on Transgene Expression in Cell Culture. J. Pharm Sci., 93, 108-23.
(2003) Kueltzo, L.A. and Middaugh, C.R. “Nonclassical Transport of Proteins and Peptides: An Alternative to Classical Macromolecular Delivery Systems.” J. Pharm. Sci., 92, 1754-72.
(2003) Lobo, B.A., Vetro, J.A., Suich, D.M., Zuckerman, R.N. and Middaugh, C.R. “Structure/Function Analysis of Peptoid/Lipitoid: DNA Complexes.” J. Pharm. Sci., 92, 1905-18.
(2003) Tiyaboonchai, W., Woiszwillo, J., Sims, R.C. and Middaugh, C.R. Insulin Containing Polyethylenimine-Dextran Sulfate Nanoparticles. Int. J. Pharm., 255, 139-151.
(2003) Tiyaboonchai, W., Woiszwillo, J. and Middaugh, C.R. Formulation and Characterization of Polyethylenimine-Dextran Sulfate Nanoparticles. Eur. J. Pharm. Sci., 19, 191-202.
(2003) Lobo, B.A., Koe, G.S., Koe, J.G. and Middaugh, C.R.. Thermodynamic analysis of binding and protonation in DOTAP:DOPE (1:1): DNA complexes using isothermal titration calorimetry. Biophys. Chem., 104, 67-78.
(2003) Kueltzo, L.A., Osiecki, J., Barker, J., Picking, W.L., Ersoy, B., Picking, W.D. and Middaugh, C.R. Structure-Function Analysis of Invasion Plasmid Antigen C (IpaC) from Shigella flexneri. J. Biol. Chem., 278, 2792-8.
(2003) Kueltzo, L.A. and Middaugh, C.R. Structural Characterization of Bovine Granulocyte Colony Stimulating Factor: Effect of Termperature and pH. J. Pharm Sci., 92, 1793-1804.
(2003) Kueltzo, L.A., Ersoy, B., Darrington, T., Ralston, J.P. and Middaugh, C.R. Derivative Absorbance Spectroscopy and Protein Phase Diagrams as Tools for Comprehensive Protein Characterization: A bGCSF Case Study. J. Pharm. Sci., 92, 1805-30.
(2003) Choosakoonkriang, S., Koe, G.S., Smith, J.G., Middaugh, C.R. Biophysical Characterization of PEI/DNA Complexes. J. Pharm. Sci., 92, 1710-1722.
(2003) Braun, C.S., Jas, G.S., Choosakoonkriang, S., Koe, G.S., Smith, J.G., Middaugh, C.R. The Structure of DNA within Cationic Lipid/DNA Complexes. Biophys. J., 84, 1114-23.
(2002) Wiethoff, C.M. Koe, J.G., Koe, G.S. and Middaugh, C.R. The Structural Organization of Cationic Lipid/DNA Complexes. J. Biol. Chem., 277, 44980-44987.
(2003) Rexroad, J., Wiethoff, C.M., Green, A.P., Kierstead, T.D., Scott, M.O., Middaugh, C.R. Structural stability of adenovirus type 5. J. Pharm Sci., 92, 665-78.
(2003) Wiethoff, C.M., Koe, J.G., Koe, G.S. and Middaugh, C.R. A Fluorescence Study of the Structure and Accessibility of Plasmid DNA Condensed with Cationic Gene Delivery Vehicles. J. Pharm. Sci., 92, 1272-1285.
(2003) Choosakoonkriang, S., Wiethoff, C.M., Koe, G.S., Koe, J.G., Anchordoquy, T.J. and Middaugh, C.R. An Infrared Spectroscopic Study of the Effect of Hydration on Cationic Lipid/DNA Complexes. J. Pharm. Sci., 92, 115-30.
(2003) Wiethoff, C.M. and Middaugh, C.R. Barriers to Nonviral Gene Delivery. J. Pharm. Sci., 92, 203-17.
(2003) Brandau, D.T., Jones, L.S., Wiethoff, C.M., Rexroad, J., Middaugh, C.R. Thermal stability of vaccines. J. Pharm. Sci., 92, 218-31.
(2002) Rexroad, J., Wiethoff, C.M., Jones, L.S., Middaugh, C.R. Lyophilization and the thermal stability of vaccines. Cell Preservation Technology, 1, 91-104.
(2002) MacLean, D.S., Qian, Q. and Middaugh, C.R. "Stabilization of Proteins by Low Molecular Weight Multi-Ions." J. Pharm. Sci., 91, 2220-2229.
(2002) Souillac, P.O., Costantino, H.R., Middaugh, C.R. and Rytting, J.H. “Investigation on Protein/Carbohydrate Interactions in the Dried State. 1. Calorimetric Studies.” J. Pharm. Sci., 91, 206-216.
(2002) Tatulian, S.A., Chen, B., Li, J., Negash, S., Middaugh, C.R., Bigelow, D.J. and Squier, T.C. “Structural Interactions Between the Ca-ATPase and Phospholamban Identified by Attenuated Total Reflection FTIR Spectroscopy.” Biochemistry, 41, 741-751.
(2002) Lobo, B.A., Rogers, S.A., Choosakoonkriang, S., Bogdanowich-Knipp, S., Smith, J.G., Koe, G. and Middaugh, C.R. “Alteration of the Thermal Stability of Plasmid DNA by Cationic Lipids and Polymers.” J. Pharm. Sci., 91, 454-466.
(2002) Volkin, D.B., Sanyal, G., Burke, C.J. and Middaugh, C.R. “Preformulation Studies as an Essential Guide for Formulation Development and Manufacture of Protein Pharmaceuticals. Pharm. Biotech, Vol. 1-4, 1-46.
(2002) Huang, C.-Y., Ma, S.S., Lee, S., Radhakrishnau, R., Braun, C., Choosakoonkriang, S., Wiethoff, C., Lobo, B. and Middaugh, C.R. "Enhancements in Gene Expression of Plasmid DNA Formulations Containing Neutral Polymeric Excipients." J. Pharm. Sci., 91, 1371-1381.
(2001) Edwards, K.L.T., Kueltzo, L., Fisher, M.T. and Middaugh, C.R. “Complex Effects of Molecular Chaperones on the Aggregation and Refolding of Fibroblast Growth Factor-1, Arch. Biochem. Biophys., 393, 14-21.
(2001) Wiethoff, C.M., Smith, J.G., Koe, G.S. and Middaugh, C.R. “The Potential Role of Proteoglycans in Cationic-Lipid Mediated Gene Delivery: Studies of the Interaction of Cationic Lipid/DNA Complexes with Model Glucosaminoglycans”, J. Biol. Chem., 275, 32806-32813.
(2001) Choosakoonkriang, S., Wiethoff, C.M., Anchordoquy, T.J., Koe, G.S., Smith, J.G. and Middaugh, C.R. “Infrared Spectroscopic Characteristics of the Interactions of Cationic Lipids with Plasmid DNA.” J. Biol. Chem., 276, 8037-8043.
(2002) Souillac, P.O., Middaugh, C.R. and Rytting, J.H. “Investigation on Protein/Carbohydrate Interactions in the Dried State. 2. Diffuse Reflectance FTIR Studies.” Int. J. Pharm., 235, 207-218.
(2001) Harned, A.M., Probst, D.A., Sheriff, B.A., Poon, K.W.C., Hanson, P.R., Wiethoff, C.W. and Middaugh, C.R. “Ring Opening Metathesis Polymerization Strategies to Chemical and Biological Delivery Agents.” Polym, Prepr. (Am. Chem. Soc. Div. Polym. Chem.) 42, 143-144.
(2000) Kueltzo, L.A. and Middaugh, C.R. “Potential Use of Non-Classical Pathways for the Transport of Macromolecular Drugs.” Exp. Op. Invest. Drugs, 9, 2039-2050.
(2001) Tiyaboonchai, W., Woiszwillo, J. and Middaugh, C.R. “Formulation and Characterization of Amphotericin B-Polyethyleneimine-Dextran Sulfate Nanoparticles.” J. Pharm. Sci, 90, 902-914.
(2000) Kueltzo, L.A., O’Hare, P.O. and Middaugh, C.R. “Conformational Lability of Herpes Virus Protein VP22.” J. Biol. Chem., 275, 33213-33221.
(2001) Lobo, B.A., Koe, G., Smith, J.G. and Middaugh, C.R. “Isothermal Titration Calorimetric Analysis of the Interaction Between Cationic Lipids and Plasmid DNA.” Arch. Biochem. Biophys, 386, 95-105.
(2001) Grillo, A.O., Edwards, K.-L.T., Kashi, R.S., Shipley, K.M., Hu., L., Besman, M.J. and Middaugh, C.R. “Conformational Origin of the Aggregation of Recombinant Human Factor VIII.” Biochemistry, 40, 586-595.
(2001) Choosakoonkriang, S., Wiethoff, C., Kueltzo, L.A. and Middaugh, C.R. “Characterization of Synthetic Gene Delivery Vehicles by Infrared Spectroscopy.” Methods Molec. Med., 65, 285-317.
(2001) Lobo, B.A., Rogers, S.A., Wiethoff, C. “Characterization of Cationic Vector-Based Gene Delivery Vehicles Using Isothermal Titration and Differential Scanning Calorimetry.” Methods Molec. Med., 65, 319-348.
(2001) Wiethoff, C and Middaugh, C.R. “Light Scattering Techniques for the Characterization of Synthetic Gene Therapy Vectors.” Methods Molec. Med., 65, 349-376.
(2001) Braun, C.S., Kueltzo, L.A. and Middaugh, C.R. “Ultraviolet Absorption and Circular Dichroism Spectroscopy of Non-Viral Gene Delivery Complexes.” Methods Molec. Med., 65, 253-284.
