Macdonald Lab

Genetics & Genomics





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Protocols


  • DNA extraction (single fly)
  • Fly food preparation
  • Lambda HindIII ladder

    • Reagents


    Single Reagent Solutions

  • DTT, dithiothreitol (1M)
  • DTT (25 mM)
  • EDTA (0.5M, pH 8.0)
  • EDTA (8 mM)
  • Ethanol (70%)
  • KCl, potassium chloride (2M)
  • MgCl2, magnesium chloride (300 mM)
  • NaOH, sodium hydroxide (10M)
  • SDS, sodium dodecyl sulfate (20%)
  • Sodium acetate (3M, pH 5.2)
  • Tris (1M, pH 7.4)
  • Tris (1M, pH 8.0)
  • Tris (1M, pH 8.5)
  • Tris (1M, pH 8.8)
  • Triton X-100 (20%)

    • Multi-Reagent Solutions

  • NaPi, sodium phosphate buffer (1M, pH 7.2)
  • 20X SSC (pH 7.0)
  • 20X SSPE (pH 7.4)
  • 1X TE (pH 8.0)
  • 1X low-EDTA TE (pH 8.0)

    • Reaction Buffers

  • 10X OLA buffer
  • 10X OLA amplification buffer
  • 10X PCR buffer

    • Agarose Gel Work

  • EtBr, ethidium bromide (10 mg/ml)
  • 10X loading buffer
  • 50X TAE

    • Membrane/Hybridization Work

  • Denaturing buffer
  • Hybridization buffer
  • Neutralization buffer
  • Stripping buffer
  • Washing buffer